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US 2026/0166201 A1Published application
Skin substitutes comprising human skin blood and lymphatic capillaries from 3d in-vitro systems
AssigneeUniversitäT ZüRich
InventorsThomas BIEDERMANN, Silvia Agnes KLAR, Dominic RÜTSCHE, Luca PONTIGGIA, Ueli MÖHRLEN
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Abstract
The present invention relates to the production of skin substitutes with a defined ratio of blood endothelial cells (BEC), to lymphatic endothelial cells (LEC), and their use in medicine.
Claims16
A method for production of a skin substitute, said method comprising the steps:
- a. providing a skin tissue sample isolated from a mammalian subject;
- b. separating the skin tissue sample into dermis and epidermis;
- c. enzymatically degrading matrix components of the dermis to obtain a plurality of cells in suspension;
- d. in a CD31 step, sorting said plurality of cells into a CD31+ cell fraction and a CD31− cell fraction;
- e. culturing the CD31+ cell fraction yielding a plurality of CD31+ cells;
- f. in a BEC/LEC step, sorting said plurality of CD31+ cells into blood endothelial cells (BEC), and lymphatic endothelial cells (LEC) using a BEC-specific and/or LEC-specific marker;
- g. in a culturing step, culturing BEC and/or LEC yielding a skin substitute.
4 more elements
Dependencies are inferred from the claim text.