Attenuation of cytokine release syndrome in immunotherapy

A recombinant nucleic acid molecule comprising:

• i. at least one hairpin loop structure formed by one or more short hairpin RNA sequences, the at least one hairpin loop structure regulating an amount of Granulocyte monocyte colony stimulating factor (GMCSF) cytokine during immunotherapy, the short hairpin RNA is encoded by at least one of SEQ ID NO. 5-SEQ ID NO. 24 or a nucleotide sequence with at least 95% identity with at least one of the SEQ ID NO. 5-SEQ ID NO. 24;
• and
• ii. a first promoter upstream of the at least one hairpin loop structure.

An open reading frame comprising:

• i. at least one hairpin loop structure formed by one or more short hairpin RNA sequences, the at least one hairpin loop structure regulating an amount of Granulocyte monocyte colony stimulating factor (GMCSF) cytokine during immunotherapy, the short hairpin RNA is encoded by at least one of SEQ ID NO. 5-SEQ ID NO. 24 or a nucleotide sequence with at least 95% identity with at least one of the SEQ ID NO. 5-SEQ ID NO. 24;
• and
• ii. a first promoter upstream of the at least one hairpin loop structure.