Enzyme measurement method, microchamber array, kit, method for capturing raman scattering image, and method for measuring target molecule

An enzyme measurement method, comprising:

• a mixed liquid preparation step in which a mixed liquid including an enzyme and a selective substrate for the enzyme, or including a plurality of types of enzymes and a plurality of types of selective substrates corresponding to the plurality of types of the enzymes is prepared;
• an array preparation step in which a microchamber array with immobilized metal nanostructures is prepared, wherein the microchamber array has a plurality of microchambers in which metal nanostructures are immobilized;
• a mixed liquid sealing step in which each of the plurality of microchambers is sealed with the mixed liquid so that one or fewer molecules of the enzyme, or at least any one type of one or fewer molecules of the plurality of types of the enzymes is present in each of the plurality of microchambers;
• and
• a bright spot counting step in which the microchamber array with immobilized metal nanostructures is illuminated with excitation light, and the number of microchambers in which a surface-enhanced Raman scattering intensity derived from an enzyme reaction product of the selective substrate or from the selective substrate has changed to a preset value or more, or is a preset threshold value or more or the threshold value or less is measured,
• wherein the enzyme reaction product has a different structure from the selective substrate, and the enzyme reaction product and the selective substrate are distinguishable by a Raman peak.