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US 2026/0160663 A1Published application
Oblique line scanner systems and methods for high throughput single molecule tracking in living cells
AssigneeEikon Therapeutics, Inc.
InventorsEric BETZIG, Fedor ILKOV, Joshua ISAACS, Behnam ABAIE, Kevin LIN
Life of this patent
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Abstract
High Throughput Single Molecule Tracking (htSMT) systems and methods are described wherein the htSMT workflows are adapted to characterize both known and novel pathway contributions to interaction networks in live cells, such as protein signaling interaction networks.
Claims20
An apparatus for fluorescence microscopy, the apparatus comprising:
- a first optical element or assembly configured to receive a fluorescence excitation light source and produce a collimated light beam having an elongated and linear shape in an x-y plane, wherein the light beam has a uniform intensity across a longer dimension of the linear shape;
- a second optical element or assembly configured to incline the light beam relative to the z-axis in an x-z plane, wherein the second optical element is further configured to focus the light beam at a sample plane located in the x-y plane, thereby illuminating a portion of the sample plane;
- a third optical element or assembly configured to translate the light beam in the sample plane in a direction orthogonal to the longer dimension of the light beam;
- and
- a detector device configured to receive light from the illuminated sample plane, wherein the detector device forms one or more projected images based on the light received from the sample plane.
2 more elements
A microscopy system for detecting the position of a molecule, comprising:
- a stage for supporting a sample, wherein the sample contains the molecule;
- a light source for emitting a light beam capable of inducing a light-based response from the molecule in the sample, wherein the light beam has a linear shape in a sample plane and has a uniform intensity across the longer dimension of the linear shape in the sample plane;
- an objective lens for focusing the light beam on the sample in the sample plane, wherein the molecule is disposed in the sample plane;
- and
- a detector device for monitoring the light-based response from the molecule, thereby detecting the position of the molecule.
2 more elements
A method for imaging one or more molecules in a sample, comprising:
- mounting a sample on a stage, the sample containing a plurality of molecules;
- illuminating a portion of a sample plane disposed within the sample with a light beam to cause fluorescence in at least a subset of the plurality of molecules in the sample, wherein the light beam has a linear shape in a sample plane and has a uniform intensity across the longer dimension of the linear shape of the light beam in the sample plane;
- detecting the fluorescence from one or more of the fluorescent molecules in the sample plane via a detector device.
Dependencies are inferred from the claim text.