A method and apparatus for rapid circulating tissue pretreatment

An automatic tissue pretreatment device for dewaxing, rehydration, epitope retrieval of biological tissue and inactivation of tissue endogenous enzymes comprising:

• a fluid tank comprising:
• a tank fluid heating element;
• a fluid tank temperature sensor;
• a low fluid level sensor,
• wherein the low fluid level sensor is installed at lower level inside of the tank to sense a certain level of tank fluid for automatically triggering or stopping any fluid heating;
• a high fluid level sensor,
• wherein the high fluid level sensor is installed at higher level inside of the tank to sense a certain level of tank fluid for automatically stopping any inflow fluid pumping;
• a vertical fluid flow guide;
• a fluid waste flow outlet;
• a horizontal fluid flow guide,
• wherein the horizontal fluid flow guide is connected to the fluid waste flow outlet;
• a fluid tank temperature controller;
• and
• a fluid inflow dispenser,
• wherein the fluid inflow dispenser is connected a fluid inflow pump, and a fluid inflow heating element, and a fluid inflow temperature sensor, and a fluid inflow temperature controller,
• wherein the fluid inflow pump, the fluid inflow heating element, the fluid inflow temperature sensor, the fluid inflow temperature controller, and the fluid inflow dispenser work together to provide temperature, time, flow rate, flow direction and fluid ingredient controls, transport and delivery of any inflow fluid.