Methods for the improved production of psilocybin and intermediates or side products through enzyme optimization

Abstract

Provided are methods, prokaryotic host cells, expression vectors, and kits for the production of psilocybin or an intermediate or a side product thereof using at least one psilocybin production gene from Psilocybe cyanescens, Panaeolus cyanescens, Gymnopilus dilepis, or Gymnopilus junonius. Also provided are methods, prokaryotic host cells, expression vectors, and kits for the production of norbaeocystin using at least one psilocybin production gene from Psilocybe cyanescens, Panaeolus cyanescens, Gymnopilus dilepis, or Gymnopilus junonius. In certain embodiments, the prokaryotic host cell is selected from the group consisting of Escherichia coli, Corynebacterium glutamicum, Vibrio natriegens, Bacillus subtilis, Bacillus megaterium, Escherichia coli Nissle 1917, Clostridium acetobutlyicum, Streptomyces coelicolor, Lactococcus lactis, Pseudomonas putida, Streptomyces clavuligerus, and Streptomyces venezuelae.

Dependencies are inferred from the claim text.