Oxidation of steroids
AssigneeOxford University Innovation Limited
InventorsLuet Lok WONG, Wenyu CHEN
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Abstract
The present invention relates to processes for the oxidation of secosteroids using mutant Cytochrome P450 family 102A sub-family member (CYP102A) enzymes. The invention also relates to mutant CYP102A enzymes, polynucleotides encoding the enzymes, cells expressing the enzymes, transgenic animals or plants comprising the enzymes, and compounds produced by the processes.
Claims25
A process for oxidising a secosteroid, comprising the step of contacting said secosteroid with a mutant CYP102A (Cytochrome P450 family 102A sub-family member) enzyme, wherein said CYP102A enzyme comprises a heme monooxygenase domain comprising a P450 fold, and said mutant CYP102A enzyme comprises substitution(s) in the polypeptide chain of a wild-type CYP102A enzyme at one or more positions, thereby enhancing monooxygenase activity and/or altering product selectivity of the mutant enzyme.
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A mutant CYP102A (Cytochrome P450 family 102A sub-family member) enzyme, wherein said CYP102A enzyme comprises a heme monooxygenase domain comprising a P450 fold, and said mutant CYP102A enzyme comprises substitution(s) in the polypeptide chain of a wild-type CYP102A enzyme at one or more positions corresponding to amino acid residue positions 435, 82, 184, 260 and/or 72 of SEQ ID NO:
- 2, thereby enhancing monooxygenase activity and/or altering product selectivity of the mutant enzyme.
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A composition of matter comprising the compound (1S,Z)-3-(2-((1R,3aS,7aR,E)-7a-methyl-1-((2R)-5-(2-methyloxiran-2-yl) pentan-2-yl) octahydro-4H-inden-4-ylidene)ethylidene)-4-methylenecyclohexan-1-ol (i.e. 25,26-epoxy-vitamin D3) or the compound (3S,6R,E)-6-((1R,3aS,7aR,E)-4-((Z)-2-((S)-5-hydroxy-2-methylenecyclohexylidene)ethylidene)-7a-methyloctahydro-1H-inden-1-yl)-2-methylhept-4-ene-2,3-diol (i.e. 25,28-dihydroxy-vitamin D2).
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Dependencies are inferred from the claim text.