Co2-fixing microorganism

Abstract

The present disclosure relates to a microorganism that fixes carbon dioxide, presenting a new strategy capable of contributing to achieving carbon neutrality in bioprocesses through development of a CO2 fixing yeast using S. cerevisiae, wherein, to address the redox imbalance problem and increase CO2 fixation efficiency, a form-I RuBisCO-based CO2 fixation pathway was established in a xylose isomerase-based xylose metabolic strain, the fermentation rate and productivity were remarkably improved by deleting the endogenous reductase genes GRE3 and YPR1, which induce xylitol accumulation, resulting in a significant increase in a yield of ethanol production along with efficient CO2 fixation, the finally developed SJ11 YΔGΔ strain had significantly reduced xylitol accumulation, greatly improved fermentation rate, and maximized CO2 fixation efficiency and ethanol productivity compared to existing strains.

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