Dendritic cell generating apparatus and method

A method of generating dendritic cells, the method comprising:

• providing a cell culture system comprising:
• a cell culture cartridge comprising a top surface and an opposing bottom surface coupled to one another and defining a cell culture chamber therebetween, wherein the cell culture chamber comprises a plurality of zones geometrically configured to provide symmetrical fluid flow channels in the cell culture chamber and to avoid dead areas of flow within the cell culture chamber during flow of a fluid therein;
• a computer system having a central processing unit for carrying out instructions;
• one or more control systems;
• and
• one or more computer programs tangibly embodied in a non-transitory computer-readable medium for execution by the computer system;
• generating immature dendritic cells in the cell culture cartridge;
• and
• maturing the immature dendritic cells in the cell culture chamber by providing a continuous perfusion of medium into the cell culture chamber via one or more inlets of the cell culture cartridge and simultaneously removing depleted medium into a waste reservoir via one or more outlets of the cell culture cartridge to produce mature dendritic cells.