Bio-ink compositions, environmentally-sensitive objects, and methods of making the same
OwnerFiorenzo G. Omenettoinventor
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Abstract
Silkworm silk, has been processed into a variety of new material formats including, hydrogels, ultrathin films, thick films, conformal coatings, 3D porous or solid matrices, fibers and many related material formats. Silk is processed in an all water-based, room temperature, neutral pH environment, is mechanically stable, edible, and biocompatible. Given the favorable material properties, the use of silk-based inks have been used in a variety of controlled chemical and biological material fabrication on the micro- and nano-scale. Tuning of the properties of silk-based inks has led to the utilization of more diverse printing techniques and has expanded the scale and function of the printed substrates.
Claims22
-36. (canceled)
A coated article, comprising:
- a substrate forming a three-dimensional hierarchical body, the body having opposing ends on respective opposing surfaces of the body defining a first surface and a second surface;
- an array of sensing elements in contact with the first surface of the substrate forming a pattern across the first surface, the sensing elements being transformable from a first chemical-physical state to a second chemical-physical state in response to a change in one or more environmental parameter, the array of sensing elements composed of:
- (i) silk fibroin, and
- (ii) a sensing additive.
-61. (canceled)
A method of colorimetric sensing,
- contacting an array of sensing elements printed on a substrate with an environment, each of the sensing elements comprising an ink composition, the ink composition comprising silk fibroin and an environmentally-responsive dye dispersed therein, wherein the array of sensing elements undergoes a color change in response to an environmental parameter;
- and
- detecting the environmental parameter from pixel intensities or colorimetric values of the array in an image of the array.
A method of colorimetric lactate sensing,
- contacting an array of sensing elements printed on a substrate with sweat, each of the sensing elements comprising silk fibroin, lactate oxidase, peroxidase, a chromogenic dye system, and optional reference indicia, wherein the array of sensing elements undergoes a colorimetric change in response to the contacting;
- and
- determining a lactate concentration from the colorimetric change of the array.
Dependencies are inferred from the claim text.