Low-sugar influenza vaccine and methods thereof
OwnerChung-Yi WU, Chi-Huey Wong +1inventors
InventorsChung-Yi WU, Chi-Huey Wong, Jeng-Shin LEE
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to a low glycosylated influenza hemagglutinin (HA) protein and a vaccine designed to express the HA protein in vivo. The present disclosure also teaches a method for generating an immune response by utilizing the low glycosylated HA protein, which provides a broader protection across different influenza strains or lineages.
Claims30
An isolated nucleic acid, which encodes a recombinant influenza hemagglutinin (HA) comprising a signal peptide, an HA1 subunit, and an HA2 subunit, wherein the influenza HA comprises at least one glycosylation site, and the HA2 subunit is devoid of a glycosylation site.
An immunogenic composition, comprising
- a first isolated nucleic acid, encoding a first recombinant influenza hemagglutinin (HA), which comprises an amino acid sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or 100% identical to SEQ ID NO:
- 6, provided that each of the 40th, the 293rd, the 304th, and the 498th amino acids thereof does not form a glycosylation site, and the 27th amino acid thereof forms a glycosylation site;
- a second isolated nucleic acid, encoding a second recombinant influenza hemagglutinin (HA), which comprises an amino acid sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or 100% identical to SEQ ID NO:
- 11, provided that each of the 24th, the 38th, the 54th, the 61st, the 79th, the 301st, and the 499th amino acids thereof does not form a glycosylation site;
- a third isolated nucleic acid, encoding a third recombinant influenza hemagglutinin (HA), which comprises an amino acid sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or 100% identical to SEQ ID NO:
- 3, provided that each of the 318th, the 347th, the 506th, the 532nd, and the 545th amino acids does not form a glycosylation site, and the 40th amino acid forms a glycosylation site;
- and
- a fourth isolated nucleic acid, encoding a fourth recombinant influenza hemagglutinin (HA), which comprises an amino acid sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or 100% identical to SEQ ID NO:
- 18, provided that each of the 504th, the 530th, and the 543rd amino acid does not form a glycosylation site, and each of the 40th, the 316th, and the 345th, amino acids forms a glycosylation site.
A recombinant influenza hemagglutinin, comprising an amino acid sequence at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, or 100% identical to SEQ ID NO:
- 3, SEQ ID NO:
- 6, SEQ ID NO:
- 9, SEQ ID NO:
- 11, SEQ ID NO:
- 16, or SEQ ID NO:
- 18.
Dependencies are inferred from the claim text.