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US 2026/0155205 A1Published application
Digital pcr detection method and apparatus
AssigneeSniper (Suzhou) Life Technology Co.
InventorGUANG-JI SHENG
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Abstract
The present application provides a digital PCR detection apparatus, a digital PCR quantitative detection method, a multi-volume digital PCR quantitative analysis method, a digital PCR detection method, a nucleic acid detection microsphere, a preparation method of the nucleic acid detection microsphere, a kit, and a high-throughput nucleic acid detection method.
Claims15
A digital PCR detection method, comprising:
- S10, preparing a nucleic acid amplification reaction liquid to be detected;
- S20, microdropletizing the nucleic acid amplification reaction liquid to be detected to form a microdroplet array;
- S30, carrying out a polymerase chain reaction for the microdroplet array, acquiring a fluorescence curve of each microdroplet in the microdroplet array, and acquiring a dissociation curve of each microdroplet in the microdroplet array;
- and
- S40, analyzing the microdroplet array according to the fluorescence curve and the dissociation curve of each microdroplet in the microdroplet array to obtain information of a nucleic acid to be detected.
2 more elements
Dependencies are inferred from the claim text.