Method to determine impact on analytes due to cytolysis in a sample
AssigneeRadiometer Medical ApS
InventorsMichael Taagaard, Peter Frischauf
Life of this patent
Estimating the term…
Abstract
Disclosed is a method and device/apparatus for determination of the contribution from lysed cells to measurement values of an analyte present in the extracellular compartment. The method employs multiple tandem measurements of analyte and an intracellular marker from samples where different numbers of cells are lysed so as to establish the relationship between the measured value of the intracellular marker and the analyte.
Claims20
A method for determination of an impact from cell lysis on a measured concentration of an analyte present in a blood cell-containing liquid sample, the method comprising:
- A) measuring the concentration of the analyte in an extracellular phase from a sample containing blood cells and measuring an concentration of an intracellular marker in the extracellular phase of the sample,
- B) subsequently lysing a fraction of the blood cells followed by measuring a concentration of the analyte and a concentration of the intracellular marker in the extracellular phase of the sample containing the lysed cells,
- and
- C) estimating a mathematical relationship between changes in the analyte concentration and the concentration of the intracellular marker at least on the basis of the pairs of measurements obtained in steps A-B,
- whereby the impact by cell lysis on measured concentrations of the analyte in samples is determined by calculating a part of the analyte concentration derived from cell lysis from a measured concentration of the intracellular marker.
A device for determination of analyte concentration distribution in an extracellular phase of a sample, wherein the device is configured to measure a total concentration of the analyte in the extracellular phase and measure a concentration of an intracellular marker in the extracellular phase, and subsequently estimate a contribution from lysis of cells to the total concentration of the analyte by inter- or extrapolation from the measured concentration of the intracellular marker based on a mathematical or functional relationship between analyte concentration and concentration of the intracellular marker.
Dependencies are inferred from the claim text.