Catlytically modified enzyme-based biosensors and methods of production thereof
AssigneeAgricul Tural University of Athens
InventorsNikolaos Labrou, Elisavet Ioannou
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Abstract
The present invention relates to catalytically modified enzyme-based biosensors comprising supramolecular protein assemblies of glutathione S-transferase (GST) and methods of production thereof. Further, the invention relates to applications of said biosensors in detecting and determining the presence of pollutants, preferably herbicides in water and kits suitable for such detection.
Claims10
A biosensor comprising a glutathione S-transferase homohexamer, wherein each transferase monomer of said homohexamer comprises a Phenylalanine-Glycine-Glycine tag at its N-terminal site, wherein said N-tagged monomer is assembled to a homohexamer through binding of Cucurbit[n]uril, preferably Cucurbit[8]uril, to said Phenylalanine-Glycine-Glycine tag and wherein said homohexamer is further characterised that it is immobilised on a polymer carrier.
Dependencies are inferred from the claim text.