Liquid chromatography-based process for the characterization and quantification of double-stranded nucleic acid

Abstract

The present disclosure is directed to a method of quantifying and characterizing double-stranded nucleic acids from a mixture comprising one or more additional nucleic acids or impurities. In some aspects, the method of the present disclosure comprises the steps of separating double-stranded nucleic acids from the mixture, digesting the double-stranded nucleic acids into nucleosides, separating nucleosides by performing at least one step of liquid chromatography, and determining concentration of double-stranded nucleic acids in the mixture. In some embodiments, the liquid chromatography is reverse phase liquid chromatography (RP-LC). In some embodiments, the method further comprises performing at least one step of ion pair reverse phase liquid chromatography (IP-RP-LC) after separating double-stranded nucleic acids from the mixture and before digesting the double-stranded nucleic acids into nucleosides.

Dependencies are inferred from the claim text.