Ultrabright fluorescent nanocomposite structures for enhanced fluorescent bioassays
AssigneeAuragent Bioscience, LLC
InventorsClare Kercher, Qisheng Jiang, Scott L. Click
Life of this patent
Estimating the term…
Abstract
Described herein is a fluorescent nanocomposite. The fluorescent nanocomposite structure may include a plasmonic nanostructure comprising having at least one localized surface plasmon resonance wavelength (λLSPR), at least one spacer coating, at least one fluorescent agent having a maximum excitation wavelength (λEX), and at least one peptide-loaded major histocompatibility complex (MHC) molecule (pMHC). The fluorescent nanocomposite structure has a fluorescent intensity that is at least 500 times greater than a fluorescent intensity of the at least one fluorescent agent alone.
Claims14
A fluorescent nanocomposite structure comprising:
- a plasmonic nanostructure having at least one localized surface plasmon resonance wavelength (λLSPR);
- at least one spacer coating;
- at least one fluorescent agent having a maximum excitation wavelength (λEX);
- and
- at least one peptide-loaded major histocompatibility complex (MHC) molecule (pMHC),
- wherein the fluorescent nanocomposite structure has a fluorescent intensity that is at least 500 times greater than a fluorescent intensity of the at least one fluorescent agent alone.
A method of identifying T-cells with specific T-cell receptors, the method comprising:
- providing a sample containing T-cells;
- contacting the sample containing T-cells with a fluorescent nanocomposite structure comprising at least one major histocompatibility complex (MHC) molecule loaded with a peptide (pMHC) that can specifically bind to T-cells containing receptors specific to the peptide;
- spatially separating the T-cells;
- exciting the fluorescent nanocomposite structure with a wavelength of light that will induce fluorescence emission;
- and
- detecting the T-cells that are labeled with the fluorescent nanocomposite structure.
Dependencies are inferred from the claim text.