Cryostorage Device
AssigneeOverture Life, Inc.
InventorsPablo Carasa Ruiz, Luis Morgado Garcia, Lionel Matthys, Cristina Dosda Munuera
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Abstract
Disclosed herein are cryostorage devices, systems, and methods for cryopreservation or vitrification of biological materials, such as oocytes and embryos. These cryostorage devices can include a capillary straw with the dual functionality for loading/unloading a sample. The devices can also include a self-sealing mechanism and an adapter for coupling with pipettes to enable loading of a predetermined volume of sample. The devices can also include a removable cap to protect the capillary during long-term cryostorage. Methods described herein relate to the manual or automated use of such devices.
Claims18
A method of cryopreservation of a biological sample, comprising:
- a) aspirating the biological sample into a cryostorage device, wherein the cryostorage device comprises:
- i) a capillary element that comprises an elongated hollow portion between a bottom opening and a top opening;
- and
- ii) a hollow body that comprises a lower end, an upper end, and a self-sealing valve, wherein the self-sealing valve is at the upper end, wherein the hollow body is connected to the top opening of the elongated hollow portion of the capillary element through the lower end of the hollow body,
- wherein the aspirating the biological sample into the cryostorage device comprises aspirating the biological sample into the elongated hollow portion of the capillary element by applying pressure to the hollow body;
- and
- b) cryopreserving the biological sample in the elongated hollow portion of the capillary element.
Dependencies are inferred from the claim text.