Human tryptophanyl-trna synthetase transgenic mouse models
AssigneesThe University of Hong Kong, Centre for Virology, Vaccinology and Therapeutics Limited
InventorsLee Lee TENG, Sze Ming Kenneth LI, Man Lung YEUNG, Lilong JIA
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Abstract
The present disclosure relates to animal models that express human tryptophanyl-tRNA synthetase (hWARS) knock-in. These animal models are highly susceptible to enterovirus infection, including but not limited to EV-D68 and EV-A71 infection. Also disclosed are screening methods and compounds that utilize these animal models.
Claims15
A transgenic mouse comprising a hWARS vector that expresses at least a portion of a hWARS gene and functional/structural variants thereof, in one or more tissues and exhibits an increased susceptibility to enterovirus infection as compared to a mouse without the hWARS gene.
A vector comprising a CMV promoter-Kozak-human WARS CDS-rBG pA cassette.
A method of screening for compounds that treat enterovirus infection comprising:
- (i) administering the compound to a transgenic mouse that expresses a portion of a hWARS gene and functional/structural variants thereof, in one or more tissues with an increased susceptibility of enterovirus infection as compared to that in a wild-type mouse, wherein the mouse that comprises a hWARS knock-in vector;
- and (ii) determining the effect of the compound on treating enterovirus infection in the transgenic mouse, relative to a control mouse not treated with the compound or a control mouse without the hWARS knock-in vector treated with the compound;
- and (iii) identifying the compound.
Dependencies are inferred from the claim text.