Flower initation markers
AssigneePhylos Bioscience, Inc.
InventorsErica Bakker, Alisha Holloway
Life of this patent
Estimating the term…
Abstract
Provided herein is the identification of markers and genes associated with flower initiation in Cannabis. The markers are useful for breeding Cannabis plants that initiate flowering at longer daylengths, which allow for earlier maturation and harvesting and ability to grow outdoors at different latitudes or in greenhouses without artificial light deprivation. Also provided are methods for selecting plants by obtaining nucleic acids and detecting one or more markers that indicate flower initiation at increased daylight hours in order to establish plant lines having shorter harvest period as a result of flowering at increased daylight hours.
Claims29
A method for producing one or more Cannabis plants that flower at increased daylight hours, the method comprising:
- (i) obtaining a nucleic acid sample from a Cannabis plant or its germplasm;
- (ii) detecting one or more markers that indicate flower initiation at increased daylight hours;
- (iii) crossing the Cannabis plant comprising the one or more markers;
- and
- (iv) obtaining one or more progeny plants comprising the one or more markers and that initiate flowering at increased daylight hours;
- wherein increased daylight hours is a light period of 13 to 18 hours.
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A method of producing one or more Cannabis plants having modified flowering, comprising:
- (i) obtaining a nucleic acid sample from a Cannabis plant or its germplasm;
- (ii) detecting one or more nucleic acid polymorphisms associated with flowering at increased daylight hours or flowering in a fewer number of days in one or more of:
- (a) EARLY FLOWERING 9 (ELF9);
- (b) FLOWERING LOCUS T (FT);
- (c) CYCLIC DOF FACTOR 2 (CDF2);
- (d) ARGONAUTE 5 (AGO5);
- (e) GAST1 PROTEIN HOMOLOG 4 (GASA4);
- (f) CLP-SIMILAR PROTEIN 3 (CLPS3);
- and
- (g) PISTILLATA (PI);
- (iii) crossing the Cannabis plant comprising the one or more nucleic acid polymorphisms, and
- (iv) obtaining progeny plants comprising the one or more nucleic acid polymorphisms, thereby producing one or more Cannabis plants having modified flowering.
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A method of producing a genetically engineered Cannabis plant that initiates flowering at increased daylight hours, comprising:
- introducing a genetic modification in ELF9 and FT, or
- introducing a beneficial allele of ELF9 and FT.
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Dependencies are inferred from the claim text.