Construction method for humanized antibody sequence evaluation model and use thereof

Abstract

The present application provides a construction method for a humanized antibody sequence evaluation model and a use thereof. The construction method for a humanized antibody sequence evaluation model comprises: acquiring amino acid sequences of a plurality of human-derived antibody templates and numbering the amino acid sequences; calculating an entropy value at each number position; and constructing a humanized antibody sequence evaluation model on the basis of the entropy value. A method for obtaining a candidate humanized antibody sequence by means of evaluation comprises: numbering a humanized antibody sequence to be evaluated; determining the weight and the probability of amino acid occurrence at each number position in the humanized antibody sequence; on the basis of the weight and the probability of amino acid occurrence, evaluating the humanized antibody sequence by using the humanized antibody sequence evaluation model constructed by means of the described method, to obtain an evaluation value; and if the evaluation value meets a preset condition, determining the humanized antibody sequence corresponding to the evaluation value as a candidate humanized antibody sequence. The present application further provides a monoclonal antibody humanization method.

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