Origami-mediated chemical agent (orca) detection platform
AssigneeBattelle Memorial Institute
InventorsMatthew Neal, Nickolas R. Andrioff, Miguel D. Pedrozo
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Abstract
Compositions and methods are provided for a deoxyribonucleic acid (DNA) based detection and/or sequestration system and methods of using that system to detect or sequester target molecules. More particularly, a detection composition is disclosed comprising an origami nucleic acid scaffold and a target binding nucleic acid aptamer integrated in the structural scaffold, such that upon binding of the target binding ligand to its target, the origami scaffold produces a signal.
Claims17
A DNA nanostructure composition comprising:
- i) a single stranded DNA scaffold and ii) an aptamer linked to said DNA scaffold, wherein the aptamer specifically binds to an organophosphorus compound, wherein the the binding of the organophosphorus compound to the aptamer causes a conformation change to the single stranded DNA scaffold resulting in the production of a detectable signal.
A DNA nanostructure composition comprising:
- i) a single stranded DNA scaffold;
- ii) a plurality of staple oligonucleotides;
- iii) an aptamer that specifically binds to a target moiety;
- iv) a donor and acceptor fluorophore of a fluorescence resonance energy transfer (FRET) pair, wherein said staple oligonucleotides are designed to fold the DNA scaffold into two distinct planar structures that are linked together at a first end of each of the first and second planar structures, wherein the first and second planar structures are free to move relative to one other in a clamshell motion, and the opposing interior surface of the first and second planar structures are populated with said donor and acceptor fluorophores, respectively;
- said aptamer is releasably linked to the first and second planar structures along a peripheral edge of the first and second planar structures, to hold the DNA scaffold in a conformation that allows for FRET transfer between the donor and acceptor fluorophores, said aptamer designed to be released from binding to both the first and second planar structures upon binding to the target moiety.
A method of sequestering an organophosphorus compound, said method comprising contacting an organophosphorus compound with an origami nucleic acid scaffold comprising
- an aptamer that specifically binds to an organophosphorus compound, said aptamer being linked to said origami nucleic acid scaffold, wherein binding of the organophosphorus compound to said aptamer causes a conformation change in the origami nucleic acid scaffold that sequesters the organophosphorus compound within, or closely associated with, the origami nucleic acid scaffold.
Dependencies are inferred from the claim text.