Methods And Systems For Dynamic Range Enhancement Of Fluorescence Detectors
OwnerJulian James Irwin, Wesley Glen Smithinventors
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Abstract
Methods and apparatuses dynamic range enhancement of fluorescence detectors are described herein. In one aspect, a method can include collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals; based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample; determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample; and based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.
Claims28
A method, comprising:
- collecting, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals;
- based on reception times associated with emission signals of the plurality of emission signals, determining a lifetime estimation for the sample;
- determining, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample;
- and
- based on the determined pileup factor, adjusting a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.
An apparatus, comprising:
- a computing device comprising:
- at least one processor;
- and
- memory configured to store computer-executable instructions that, when executed by the at least one processor, cause the apparatus to:
- collect, from a sample during a sample event, an emission spectrum that comprises a plurality of emission signals;
- based on reception times associated with emission signals of the plurality of emission signals, determine a lifetime estimation for the sample;
- determine, based at least on the lifetime estimation, a pileup factor affecting receiving emissions from the sample;
- and
- based on the determined pileup factor, adjust a number of emission signals measured during the sample event to generate an estimated number of emissions for the sample event.
Dependencies are inferred from the claim text.