Lysis devices having a piezo element and methods

An analyzer, comprising:

• a device having:
• a sample vessel having a microchannel formed therein, the sample vessel having at least one port extending through a surface to the microchannel;
• at least one piezo element attached to the sample vessel;
• a controller having logic to cause the controller to emit a first signal including a series of frequencies to the at least one piezo element to cause the at least one piezo element to generate first acoustic standing waves in the sample vessel, to receive a second signal indicative of measured vibration signals from the sample vessel detected by the at least one piezo element, determine a resonant frequency of the sample vessel using the measured vibration signals and drive the at least one piezo element with a signal at a determined resonant frequency to generate second acoustic standing waves;
• and,
• an absorbance spectrophotometer comprising a transmitter and a receiver positioned adjacent to the sample vessel, the transmitter positioned to emit a light medium through the microchannel, and a receiver positioned to receive at least a portion of the light medium after the portion of the light medium has passed through the microchannel.

A method, comprising:

• generating, by at least one piezo element receiving a first signal, acoustic standing waves during a first frequency sweep for a first duration of time in a sample vessel;
• receiving, by a controller, a second signal indicative of vibration from the sample vessel due to the first signal;
• comparing, by the controller the first signal and the second signal to determine resonant frequency of the sample vessel;
• passing a whole blood sample into a microchannel of the sample vessel;
• and,
• emitting, by the at least one piezo element, acoustic standing waves based on a determined resonant frequency.