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US 2026/0146299 A1Published application
Electrochemical Genosensor Based on Target-Triggered DNAzyme Activity
AssigneeThe Penn State Research Foundation
InventorsDipanjan Pan, Maha Mohammad Shehadeh Alafeef
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Abstract
Embodiments relate to methods and processes for screening and detecting infectious diseases. Embodiments further relate to a molecularly driven (nucleic acid) method and process for screening and detecting infectious diseases using an electrochemical genosensor with a highly specific cleavage-triggered DNA polymerization reaction.
Claims22
A method for detecting an infectious disease, comprising:
- introducing a sample comprising RNA and/or DNA collected from a subject to a reagent solution to form a reaction mixture, wherein the reagent solution comprises at least one DNAzyme configured to target and amplify an RNA and/or DNA expression profile linked to a pre-selected infectious disease;
- and
- adding an oxidizing solution to the reaction mixture to form a detection mixture, wherein the oxidizing solution comprises at least one organic substrate and hydrogen peroxide.
An apparatus for detecting an infectious disease, comprising:
- a genosensor, wherein the genosensor comprises:
- a housing having (a) a reagent solution retaining vessel having a reagent solution comprising at least one DNAzyme configured to target and amplify an RNA and/or DNA expression profile for a pre-selected infectious disease and (b) an oxidizing solution retaining vessel having an oxidizing solution comprising at least one organic substrate and hydrogen peroxide;
- the housing configured to receive a sample having RNA and/or DNA collected from a subject for mixing with the reagent solution and the oxidizing solution for depositing on a sensor strip positionable within the reagent housing.
A method for detecting an infectious disease, comprising:
- mixing a sample comprising RNA and/or DNA collected from a subject with a reagent solution and an oxidizing solution to form a detection mixture, wherein the reagent solution comprises at least one DNAzyme, wherein the DNAzyme is configured to target and amplify an RNA and/or DNA expression profile linked to a pre-selected infectious disease;
- and
- determining whether an oxidation reaction occurs within the detection mixture to determine whether the pre-selected infectious disease is present in the sample such that a detection of the oxidation reaction indicates the pre-selected infectious disease is present and a non-detection of the oxidation reaction indicates the pre-selected infectious disease is not present.
Dependencies are inferred from the claim text.