Method for one-pot biosynthesis of arabinonucleoside, and composition

A method for one-pot biosynthesis of an arabinonucleoside, comprising:

• mixing a substrate, a purine nucleoside phosphorylase and any one of the following together:
• a uracil nucleoside phosphorylase or a pyrimidine nucleoside phosphorylase, and, then performing the one-pot biosynthesis, and directly obtaining the arabinonucleoside, wherein
• the uracil nucleoside phosphorylase comprises UP, and the UP is a protein shown as SEQ ID NO:
• 1;
• or a protein that is of more than 80% identity with the UP and has the same function;
• the pyrimidine nucleoside phosphorylase comprises PyNP, and the PyNP is a protein shown as SEQ ID NO:
• 2;
• or a protein that is of more than 80% identity with the PyNP and has the same function;
• the purine nucleoside phosphorylase comprises PNP, and the PNP is a protein shown as SEQ ID NO:
• 3;
• or a protein that is of more than 80% identity with the PNP and has the same function;
• and
• the substrate comprises a 1-beta-D-Arabinofuranosyluracil, a substrate base and a substrate phosphate.