Methods for reprogramming and gene editing cells

Abstract

The present disclosure provides improved methods for reprogramming and gene editing cells. In one aspect, the present disclosure provides a method of introducing a single strand break in a DNA site. In one aspect, the present disclosure provides a method of inserting a sequence in a DNA site by introducing a single strand break followed by insertion of the sequence using a single-stranded repair template. In another aspect, the present disclosure provides a method of activating gene expression in a cell during its differentiation. In another aspect, the present disclosure methods and compositions related to iPSC-derived mesenchymal stroma/stem cells that overexpressed IDO1. In another aspect, the present disclosure provides a solid support comprising iPSC-derived mesenchymal stem cells which can be used to treat organ damage.

Dependencies are inferred from the claim text.