Methods of lipid nanoparticle production in cross-mixers

A method, comprising:

• feeding an mRNA solution to a first inlet, the first inlet splitting into a first sub-inlet and a second sub-inlet, the first sub-inlet connected to a first side of a central region of a mixer, the second sub-inlet connected to a second side of the central region of the mixer;
• feeding an eLNP solution to a second inlet, the second inlet connected to a third side of the central region of the mixer, the third side orthogonal to the first side and the second side;
• mixing the mRNA and the eLNP in the central region to form encapsulated eLNP;
• and
• transferring the encapsulated eLNP out of the central region via an outlet, the outlet coupled to a fourth side of the central region, the fourth side opposite the third side.

A method, comprising:

• feeding an mRNA solution to a first inlet in a first direction;
• feeding an eLNP solution to a second inlet in a second direction, the second direction approximately 180 degrees offset from the first direction;
• splitting the mRNA solution into a first sub-stream and a second sub-stream;
• feeding the mRNA solution into a mixing chamber via the first sub-stream and the second sub-stream;
• feeding the eLNP solution to the mixing chamber via the second inlet;
• and
• mixing the mRNA solution and the eLNP solution in the mixing chamber to form encapsulated eLNP.