Methods for promoting homing and engraftment of hematopoietic stem cells
OwnerReuben Kapur, Zuepeng Wang +1inventors
InventorsReuben Kapur, Zuepeng Wang, Broxmeyer Hal
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Abstract
Methods to improve homing and engraftment of hematopoietic stem cells, particularly cord blood CD34+ cells, for use in hematopoietic cell transplantation through the regulation of expression of YTHDF2 or FTO in CD34+ cells. The methods include transiently repressing expression of YTHDF2 through exposure of the CD34+ cells to YTHDF2 repressor compound. The methods also include exposing CD34+ cells to a FTO expression activator compound to transiently increase FTO expression in the cells.
Claims24
A method of preparing hematopoietic stem cells for homing during stem cell transplantation, comprising
- a. contacting hematopoietic stem cells with a YTHDF2 repressor compound for about 8 to about 48 hours;
- and
- b. removing the YTHDF2 repressor compound from contact with the hematopoietic stem cells prior to transplantation of the hematopoietic stem cells into a subject.
A method of preparing hematopoietic stem cells for homing during stem cell transplantation, comprising
- a. contacting hematopoietic stem cells with an FTO expression activator compound for about 8 to about 48 hours;
- and
- b. removing the FTO expression activator compound from contact with the hematopoietic stem cells prior to transplantation of the hematopoietic stem cells into a subject.
A method of treatment of cord blood cells in preparation for homing, comprising
- a. contacting cord blood cells with a YTHDF2 repressor compound for about 6 to about 48 hours;
- and
- b. removing the YTHDF2 repressor compound from contact with the cord blood cells prior to transplantation of the cord blood cells into a subject.
Dependencies are inferred from the claim text.