Compositions and methods for inducing oocyte maturation
AssigneeGameto, Inc
InventorsChristian KRAMME, Dina Radenkovic, Martin Varsavsky, Klaus Wiemer
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Abstract
Featured are methods, compositions, and apparatuses for the in vitro maturation of oocytes. In particular, the disclosure features methods of inducing oocyte maturation in vitro, by co-culturing a female subject's oocytes with an ex vivo composition containing a plurality of ovarian support cells (e.g., granulosa cells). Additional methods for administering follicular triggering agents and retrieving oocytes from the female subject are provided. Such methods, compositions, and apparatuses are particularly useful for assisted reproduction technology (ART) procedures.
Claims30
A method of inducing in vitro maturation of one or more oocytes that have previously been retrieved from a human subject, the method comprising co-culturing the one or more oocytes with a population of ovarian support cells.
An ex vivo composition comprising a population of ovarian support cells, one or more diluents or excipients, and one or more GV-stage or MI-stage stage oocytes, optionally wherein the population comprises from about 10,000 to about 100,000 ovarian support cells.
An ex vivo cell culture medium comprising a population of ovarian support cells and one or more GV-stage or MI-stage oocytes, optionally wherein the population comprises from about 10,000 to about 150,000 ovarian support cells.
Dependencies are inferred from the claim text.