Systems and methods for cell culturing
AssigneeFlaskworks, LLC
InventorsShashi K. Murthy, Andrew Kozbial
Life of this patent
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Abstract
Cell culture systems and methods provide improved immunotherapeutic product manufacturing with greater scalability, flexibility, and automation. Cell culture systems are configured with interchangeable cartridges, allowing versatility and scalability. Systems are configured to have multiple connected cell culture chambers, which allows parallel processing of different types of cells. Gas-impermeable cell culture chambers and methods for generating cells in closed systems prevent contamination and user error. Methods for recycling cell culture medium provide additional efficiencies.
Claims13
A method for producing neo-antigen transduced T cells, the method comprising:
- providing a cell culture instrument comprising first and second culture chambers;
- flowing cell culture medium containing monocytes into the first culture chamber;
- perfusing the purified monocytes in the first culture chamber to produce dendritic cells in the first culture chamber;
- contacting the dendritic cells with antigen material in the first culture chamber to produce mature dendritic cells;
- flowing a cell suspension comprising T cells into the first culture chamber to co-culture the mature dendritic cells and the T cells, to thereby produce expanded neoantigen-targeting T cells;
- wherein the method is performed on a single instrument in a closed manner such that sterility is maintained throughout the method.
Dependencies are inferred from the claim text.