Modular Strip-Well Assay Kits with Preloaded Reagents

An assay kit comprising:

• a modular strip-well plate including a plurality of detachable strips, each strip comprising a plurality of wells;
• at least one well containing a premeasured set of mutually reactive reagents co-located in the same well;
• a sequestration structure within said at least one well configured to maintain separation of at least one reagent from at least one other reagent during storage, the sequestration structure comprising encapsulation of at least one reagent or spatial separation of reagents within the well;
• and
• a removable seal covering said at least one well;
• wherein, upon addition of an aqueous sample, buffer, or water to said at least one well, said sequestration structure releases to permit said reagents to mix in the same well to form a reaction mixture that yields a detection signal;
• and
• wherein the kit is ready-to-use and configured to operate without transfer of reagents between wells prior to reading said detection signal.