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US 2026/0145159 A1Published application
Mixed mode charge induction ion exchange (iex) chromatography (mmciic) ligands and methods of use
AssigneeBio-Rad Laboratories, Inc.
InventorsCHRISTOPHER BELISLE, ALEXANDER POLGAR, GREGORY SHIMAMURA, KANGLING MA
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Abstract
The subject invention pertains to mixed mode charge induction IEX chromatography (MMCIIC) ligands and chromatography resins suitable for the purification of proteins from biological sources or biological samples. Methods of making mixed mode charge induction IEX chromatography (MMCIIC) matrices comprising the disclosed ligands and using the disclosed chromatography matrices are also provided.
Claims20
A ligand of the structure:
- where X is CH or N;
- or
- and for each ligand:
- and the * represents a stereogenic carbon atom.
1 more element
A mixed mode charge induction IEX chromatography (MMCIIC) chromatography medium comprising a solid support and a ligand and having the formula:
- where n represents an alkyl group of 1 to 10 carbon atoms covalently bonded to the solid support (sphere) and the nitrogen atom, X is CH or N;
- or
- where n represents an alkyl group of 1 to 10 carbon atoms covalently bonded to the solid support (sphere) and the nitrogen atom;
- or
- where Y represents an alkyl linker that is a straight or branched, saturated, aliphatic divalent radical having between 1-10 carbon atoms that is covalently bonded to the solid support (sphere) and nitrogen atom, X is CH or N;
- or
- where Y represents an alkyl linker that is a straight or branched, saturated, aliphatic divalent radical having between 1-10 carbon atoms that is covalently bonded to the solid support (sphere) and nitrogen atom;
- and
- and the * representing a stereogenic carbon atom.
6 more elements
Dependencies are inferred from the claim text.