Skin tissue
AssigneeThe University of Tokyo
InventorsNaoaki MIZUNO, Hiromitsu NAKAUCHI, Hisato NAGANO, Hideki MASAKI, Motoo WATANABE, Hideyuki SATO
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Abstract
The present disclosure provides an isolated skin tissue comprising an epidermis, a basement membrane, and a dermis, wherein the epidermis is attached to one surface of the basement membrane, and the dermis is attached to the other surface of the basement membrane; and the epidermis comprises first cells, and the dermis comprises second cells, wherein the second cells have no ability to differentiate into mature epidermal cells.
Claims11
An isolated skin tissue comprising an epidermis, a basement membrane, and a dermis, wherein the epidermis is attached to one surface of the basement membrane, and the dermis is attached to the other surface of the basement membrane;
- and the epidermis comprises first cells, and the dermis comprises second cells, wherein the second cells have low or no ability to differentiate into mature epidermal cells.
A method for preparing a skin tissue, comprising:
- providing a host embryo incapable of autonomously developing a mature epidermis;
- injecting donor cells having ability to differentiate into a mature epidermis to the host embryo, wherein the injection is performed under conditions suitable for engraftment of the cells to the skin;
- allowing the obtained embryo to grow in the uterus of a pseudo-pregnant surrogate mother mammal by grafting to the uterus thereof to obtain an individual having a mature epidermis from the mammal;
- and
- isolating a skin tissue comprising a mature epidermis comprising cells derived from the donor cells, a basement membrane, and a dermis comprising cells derived from the host from the obtained individual.
A method for preparing a nonhuman mammalian individual, comprising bringing cells having ability to form a chimera and having ability to differentiate into a mature epidermis, or cells committed to an epidermis or cells of epidermal lineage (donor cells) into contact with a body surface of a nonhuman mammalian individual (host) before formation of epidermal basal cells, thereby obtaining a nonhuman mammalian individual having a skin tissue comprising cells of the host and the donor cells.
Dependencies are inferred from the claim text.