System, method, and article for detecting abnormal cells using multi-dimensional analysis

A method of detecting leukemias in a patient, the method comprising, comprising:

• exposing each cell in a normal set of biological cells to a plurality of four or more reagents using a first protocol;
• measuring a corresponding plurality of fluorescence intensities of each cell in the normal set of biological cells using a second protocol;
• mapping each cell in the normal set of biological cells to a corresponding point in an n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the normal set of biological cells, wherein the corresponding points form a normal set of points;
• defining one or more reference populations in the normal set of points using support vector machines;
• defining a set of reference clusters in the n-dimensional space by defining a centroid line and radii based at least in part on the defined one or more reference populations, wherein each cluster in the set of reference clusters corresponds to a maturation level within a cell lineage;
• exposing each cell in a test set of biological cells drawn from the patient to the plurality of reagents using the first protocol;
• measuring a corresponding plurality of fluorescence intensities of each cell in the test set of biological cells using the second protocol;
• mapping each cell in the test cell of biological cells to a corresponding point in the n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the test set of biological cells, wherein the corresponding points form a test set of points;
• comparing the test set of points to the set of reference clusters;
• and
• detecting leukemias in the patient based on the comparing of the test set of points to the set of reference clusters.

A non-transitory computer readable media storing instructions to cause a computing system to perform a method, the method comprising:

• exposing each cell in a normal set of biological cells to a plurality of four or more reagents using a first protocol;
• measuring a corresponding plurality of fluorescence intensities of each cell in the normal set of biological cells using a second protocol;
• mapping each cell in the normal set of biological cells to a corresponding point in an n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the normal set of biological cells, wherein the corresponding points form a normal set of points;
• defining one or more reference populations in the normal set of points using support vector machines;
• defining a set of reference clusters in the n-dimensional space by defining a centroid line and radii based at least in part on the defined one or more reference populations, wherein each cluster in the set of reference clusters corresponds to a maturation level within a cell lineage;
• exposing each cell in a test set of biological cells drawn from a patient to the plurality of reagents using the first protocol;
• measuring a corresponding plurality of fluorescence intensities of each cell in the test set of biological cells using the second protocol;
• mapping each cell in the test cell of biological cells to a corresponding point in the n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the test set of biological cells, wherein the corresponding points form a test set of points;
• and
• comparing the test set of points to the set of reference clusters;
• and
• detecting leukemias in the patient based on the comparing of the test set of points to the set of reference clusters.

A method of detecting leukemias in a patient, the method comprising, comprising:

• exposing each cell in a test set of biological cells drawn from the patient to a plurality of reagents using a first protocol;
• measuring a corresponding plurality of fluorescence intensities of each cell in the test set of biological cells using a second protocol;
• mapping each cell in the test cell of biological cells to a corresponding point in an n-dimensional space based at least in part on the measured plurality of fluorescence intensities of the cell in the test set of biological cells, wherein the corresponding points form a test set of points;
• comparing the test set of points to a set of reference clusters;
• and
• detecting leukemias in the patient based on the comparing of the test set of points to the set of reference clusters, wherein,
• the set of reference clusters in the n-dimensional space are defined by a centroid line and radii based on one or more defined reference populations in a normal set of points,
• the one or more defined reference populations are defined using support vector machines, and
• each cluster in the set of reference clusters corresponds to a maturation level within a cell lineage.