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AssigneeUniversity of Washington
InventorsMaya Singh, Ayokunle Olanrewaju, Megan Chang, Qin Wang, Catherine Rodgers, Barry Ryan Lutz, Carrie HsuanYu Lin
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Provided herein are systems and methods for detecting a target enzyme and/or target enzyme activity. In some embodiments, the system comprises: (a) a CRISPR effector system; and (b) a nucleic acid probe comprising a detectable label. In some embodiments, the CRISPR effector system comprises: (i) at least one CRISPR effector protein having collateral cleavage activity; and (ii) at least one sgRNA that directs the at least one CRISPR effector protein to specifically bind to a target nucleic acid sequence. In some embodiments, the target nucleic acid sequence is a sequence generated/synthesized by the activity of the target enzyme. In some embodiments, the nucleic acid probe is cleavable by collateral cleavage activity of the at least one CRISPR effector protein. Also provided herein is a point of care method for monitoring adherence to a therapy targeting a disease-associated enzyme in a subject using the systems disclosed herein.
A system for detecting a target enzyme, the system comprising:
A lateral flow device/system for detecting/monitoring enzymatic activity of a target enzyme, the device/system comprising a substrate comprising a first end, wherein the first end comprises:
A point of care method for monitoring adherence to a therapy targeting a disease-associated enzyme in a subject, the method comprising:
Dependencies are inferred from the claim text.