Devices, systems and methods for biomarker analysis

A method, comprising:

• obtaining or receiving a fluid sample from a subject via a sample container of a device, wherein the fluid sample comprises a plurality of fragmented cell-free DNA molecules comprising one or more methylation markers;
• amplifying the plurality of fragmented cell-free DNA molecules with an amplification reagent on the device thereby producing a plurality of amplified fragments of cell-free DNA molecules, wherein the amplification reagent comprises multiple primer pairs;
• and
• determining a disease of the subject from the one or more methylation markers of the plurality of amplified fragments of cell-free DNA molecules.

A system, comprising:

• a device configured to collect or obtain a fluid sample from a subject via a sample container of the device, wherein the fluid sample comprises a plurality of fragmented cell-free DNA molecules comprising one or more methylation markers,
• wherein the device comprises a processor configured to perform executable instructions, and
• wherein the device comprises a program comprising instructions executable by the processor to:
• amplify the plurality of fragmented cell-free DNA molecules with an amplification reagent on the device thereby producing a plurality of amplified fragments of cell-free DNA molecules, wherein the amplification reagent comprises multiple primer pairs:
• obtain a plurality of signals corresponding to the plurality of amplified fragments of cell-free DNA molecules with a detector on the device;
• and
• determine a disease of the subject from the plurality of signals based at least on the one or more methylation markers of the plurality of amplified fragments of cell-free DNA molecules.