Adipose-derived regenerative cell preparations
AssigneeBlue Cell Therapeutics ApS
InventorsGitte Lund Christensen, Visnja Radulovic, Elena Pedraz-Cuesta, Maja Lind Nybo, Benjamin Claass, Jone Marita Kvam, Søren Paludan Sheikh, Jakob Lerche Hansen, Thomas Sandal, Reza Yarani
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Abstract
The present disclosure relates to adipose-derived regenerative cell (ADRC) preparations and methods of culturing ADRCs to obtain ASCs. In particular the present disclosure relates to adipose-derived stromal cells derived from these cultures and their use in treatment.
Claims15
A method of generating a plurality of adipose-derived stromal cells (ASCs) from adipose tissue, comprising:
- (a) providing a cell preparation derived from the adipose tissue (an adipose-derived regenerative cell (ADRC) preparation) and a culture medium comprising from about 0.5 ng/mL to about 50 ng/mL VEGF, from about 0.5 ng/ml to about 50 ng/ml EGF, from about 1 ng/mL to about 100 ng/mL bFGF, from about 2 ng/ml to about 200 ng/ml IGF, from about 0.1 μg/mL to about 10 μg/mL Ascorbic acid, and from about 0.02 μg/mL to about 2 μg/mL hydrocortisone;
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- (b) contacting the ADRC preparation with the culture medium for a time sufficient to grow mesenchymal stem cells (MSCs) in the ADRC preparation, thereby generating the ASCs.
An adipose-derived stromal cell (ASC) preparation, produced by a process comprising:
- (a) providing a cell preparation derived from adipose tissue (an adipose-derived regenerative cell (ADRC) preparation) and a culture medium comprising from about 0.5 ng/ml to about 50 ng/mL VEGF, from about 0.5 ng/ml to about 50 ng/mL EGF, from about 1 ng/ml to about 100 ng/mL bFGF, from about 2 ng/mL to about 200 ng/ml IGF, from about 0.1 μg/mL to about 10 μg/mL ascorbic acid, and from about 0.02 μg/mL to about 2 μg/mL hydrocortisone;
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- (b) contacting the ADRC preparation with the culture medium for a time sufficient to grow mesenchymal stem cells (MSCs) in the ADRC preparation, thereby producing the adipose-derived stromal cells (ASCs).
Dependencies are inferred from the claim text.