Development of multi-antigen mrna vaccine against feline fipv

An isolated nucleic acid molecule, comprising:

• a first nucleic acid fragment encoding an M protein of feline infectious peritonitis virus;
• a second nucleic acid fragment encoding an N protein of feline infectious peritonitis virus;
• and
• a third nucleic acid fragment encoding an S protein, Sec protein, or SII protein of feline infectious peritonitis virus,
• wherein the first nucleic acid fragment, the second nucleic acid fragment, and the third nucleic acid fragment are linked or not linked;
• and
• wherein the nucleic acid molecule is mRNA and is a linear molecule.

A method for constructing a feline infectious peritonitis virus vaccine, the method comprising:

• introducing a nucleic acid fragment into a recipient cell,
• wherein the nucleic acid fragment comprises at least one of a first nucleic acid fragment, a second nucleic acid fragment, and a third nucleic acid fragment, wherein:
• the first nucleic acid fragment encodes an M protein of feline infectious peritonitis virus;
• the second nucleic acid fragment encodes an N protein of feline infectious peritonitis virus;
• the third nucleic acid fragment encodes an S protein, S_ec protein, or SII protein of feline infectious peritonitis virus;
• and
• the first nucleic acid fragment, the second nucleic acid fragment, and the third nucleic acid fragment are linked or not linked,
• the method further comprising, prior to said introducing into the recipient cell:
• encapsulating the nucleic acid fragment with an encapsulation carrier,
• wherein the encapsulation carrier is selected from at least one of a liposome, an exosome, a polymer carrier, a viral vector, or a nanoparticle, preferably a liposome,
• wherein the recipient cell is a CRFK cell, HEK293FT cell, HEK293T cell, a BHK cell, or an insect cell, preferably a CRFK cell.