Method for constructing circular rna and vaccine against fipv

An isolated nucleic acid molecule, comprising:

• a first nucleic acid fragment encoding an M protein of feline infectious peritonitis virus;
• and
• a second nucleic acid fragment encoding an N protein of feline infectious peritonitis virus,
• wherein the first nucleic acid fragment is linked or not linked to the second nucleic acid fragment;
• and
• wherein the nucleic acid molecule is a circular RNA.

A method for constructing a feline infectious peritonitis virus vaccine, the method comprising:

• introducing a nucleic acid fragment into a recipient cell,
• wherein the nucleic acid fragment is a circular RNA, and the nucleic acid fragment comprises at least one of a first nucleic acid fragment, a second nucleic acid fragment, and a third nucleic acid fragment;
• wherein:
• the first nucleic acid fragment encodes an M protein of feline infectious peritonitis virus;
• the second nucleic acid fragment encodes an N protein of feline infectious peritonitis virus;
• the third nucleic acid fragment encodes an S protein, Sec protein, or SII protein of feline infectious peritonitis virus;
• and
• the first nucleic acid fragment, the second nucleic acid fragment, and the third nucleic acid fragment are linked or not linked,
• the method further comprising, prior to said introducing into the recipient cell:
• encapsulating the nucleic acid, the expression vector, or the recombinant virus with an encapsulation carrier,
• wherein the encapsulation carrier is selected from at least one of a liposome, an exosome, a polymer carrier, a viral vector, or a nanoparticle, preferably a nanoparticle,
• wherein the recipient cell is a CRFK cell, HEK293FT cell, HEK293T cell, a BHK cell, or an insect cell, preferably a CRFK cell.