Methods of treating male hypogonadism with hormone-producing organoids
OwnerCraig S. ATWOOD, Sin-Ruow TEYinventors
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Abstract
A method of treating male hypogonadism in a subject in need thereof includes administering to the subject testicular organoids generated from isolated male progenitor cells. The method can be used to slow or halt the progression of age-related diseases, and increase healthspan and longevity, in hypogonadal males. The method can be used with subjects that have not or are not receiving testosterone replacement therapy. The method does not require that the subject is pre-treated with CG. The testicular organoid includes cells expressing GFRA1, VEGF, VEGFR2, PDGF-Rα, Cyp11A1, 3β-HSD, 17β-HSD, LHCGR, α-SMA, CD11b, MHC CII, CD64, CD95, Sox9, AR, inhibin β-B, and FSHR.
Claims32
A method of treating male hypogonadism in a subject in need thereof comprising administering to the subject testicular organoids generated from isolated male progenitor cells.
A method of generating a testicular organoid, the method comprising:
- (a) generating a spheroid from isolated male progenitor cells under dynamic conditions;
- and
- (b) culturing the spheroid under dynamic conditions in a culture medium supplemented with a luteinizing hormone (LH), follicle stimulating hormone (FSH), a thyroid hormone, human insulin-like growth factor 1 (IGF-1), human glial cell line-derived neurotrophic factor (GDNF), retinoic acid, smoothened agonist (SAG), 8-bromoadenosine 3′,5′-cyclic adenosine monophosphate (8-Br-cAMP), testosterone, and platelet-derived growth factor AA or platelet-derived growth factor AB to generate the testicular organoid.
A method of increasing health span or longevity in a male subject in need thereof comprising administering to the subject testicular organoids generated from isolated male progenitor cells.
A method of rebalancing the hypothalamic-pituitary-gonadal (HPG) axis in a male subject in need thereof comprising administering to the subject testicular organoids generated from isolated male progenitor cells.
A method of slowing or halting diseases of aging in a male subject in need thereof comprising administering to the subject testicular organoids generated from isolated male progenitor cells.
Dependencies are inferred from the claim text.