Detection of fumarate-derived modification in a test sample
AssigneeUniversity of South Carolina
InventorNORMA FRIZZELL CATLEDGE
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Abstract
Examination of a test sample to determine the succination level of test sample is described. Examination can be via protein hydrolysis in total succination determination or via enzymatic digestion of isolated proteins and determination of the presence or quantity of modified peptides. The methods can be utilized for determination of excessive succination of lymph system proteins, which can be utilized in prevention or early detection of autoimmune disorders. Methods can be utilized for test samples of subjects under treatment with dimethyl fumarate suffering from an autoimmune disorder. Methods can be utilized as a determination that treatment of the subject with DMF should be slowed or stopped.
Claims18
A method for determining a succination level in a test sample, comprising:
- isolating one or more components from the test sample of a subject, wherein the test sample comprises white blood cells, red blood cells, peripheral blood mononuclear cells, platelets, total serum, urinary proteins, or a combination thereof;
- hydrolyzing proteins of the test sample;
- examining a hydrolysis product according to a negative ion mode mass spectrometry analysis technique;
- and
- by use of the examination, determining the presence or quantity of free s-(2-succino)cysteine (2SC) in the test sample,
- wherein the subject has been treated with dimethyl fumarate or a monomethyl fumarate prodrug prior to determining the presence or quantity of free 2SC in the test sample.
Dependencies are inferred from the claim text.