Nanobiosensors and methods for detection and measurement using nanobiosensors
AssigneeThe Trustees of Columbia University in the City of New York
InventorsQiao Lin, Milan Stojanovic, Shifeng Yu, Chao Su, Nenad Milosavic, Wenting Dai
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Abstract
Disclosed is a device including a transistor that includes a graphene-based conducting channel, a functional layer modified with an aptamer, and attached to the graphene-based conducting channel via a polymer, such that the aptamer binds with a target biomarker. A method for manufacturing an aptamer-based biosensor, and a method for detecting a target biomarker using the aptamer-based biosensor, are also provided.
Claims20
A device comprising:
- a transistor comprising a graphene-based conducting channel;
- and
- a functional layer modified with an aptamer, and attached to the graphene-based conducting channel via a polymer,
- wherein the aptamer binds with a target biomarker.
A method for manufacturing an aptamer-based biosensor comprising:
- creating a graphene-based conducting channel in a field effect transistor;
- coating the graphene-based conducting channel with a functional layer, wherein the functional layer is a polymer;
- and
- reacting an aptamer with the functional layer to form the aptamer-based biosensor.
A method for detecting a target biomarker comprising:
- manufacturing a device comprising a transistor with a graphene-based conducting channel;
- coating the graphene-based conducting channel with a functional layer modified with an aptamer;
- measuring a first drain-source current of the device;
- exposing the device to a medium comprising a target biomarker;
- measuring a second drain-source current of the device;
- and
- determining binding of the aptamer to the target biomarker based on a difference between the first and second drain-source currents.
Dependencies are inferred from the claim text.