CMD Linkers for Label-Free Molecular Analysis, Methods of Use and Manufacture

A sensor for label-free molecular analysis, comprising:

• a sensor surface comprising a substrate coated with a metal layer;
• and
• a linker disposed on the metal layer comprising a compound of Formula I:
• wherein comprises sulfur, carbon, nitrogen, oxygen, selenium, tellurium, or polonium,
• wherein R2 comprises carboxyl, biotin, streptavidin, neutravidin, avidin, hydrazine, aminooxy, thiol, epoxy, tetrazine, trans-cyclooctene, aryl azide, diazirine, polyethylene glycol, zwitterionic moieties, amine, aldehyde, pyridyl-disulfide, maleimide, N-hydroxysuccinimide ester, nitrilotriacetic acid, azide, alkyne, a photocleavable moiety, or a photoreactive moiety,
• wherein m is about 1 to about 6000, and
• wherein n is about 1 to about 80.

A reagent kit for treating a sensor surface, the sensor surface comprising a linker comprising a compound of Formula I:

• wherein R1 comprises sulfur, carbon, nitrogen, oxygen, selenium, tellurium, or polonium,
• wherein R2 comprises carboxyl, biotin, streptavidin, neutravidin, avidin, hydrazine, aminooxy, thiol, epoxy, tetrazine, trans-cyclooctene, aryl azide, diazirine, polyethylene glycol, zwitterionic moieties, amine, aldehyde, pyridyl-disulfide, maleimide, N-hydroxysuccinimide ester, nitrilotriacetic acid, azide, alkyne, a photocleavable moiety, or a photoreactive moiety,
• wherein m is about 1 to about 6000, and
• wherein n is about 1 to about 80,
• the reagent kit comprising:
• a first solution comprising an acid and a surfactant;
• a second solution comprising a ligand;
• and
• a third solution comprising an amine.

A method for using a reagent kit to immobilize a ligand on a linker-functionalized sensor surface comprising a compound of Formula I:

• wherein R1 comprises sulfur, carbon, nitrogen, oxygen, selenium, tellurium, or polonium,
• wherein R2 comprises carboxyl, biotin, streptavidin, neutravidin, avidin, hydrazine, aminooxy, thiol, epoxy, tetrazine, trans-cyclooctene, aryl azide, diazirine, polyethylene glycol, zwitterionic moieties, amine, aldehyde, pyridyl-disulfide, maleimide, N-hydroxysuccinimide ester, nitrilotriacetic acid, azide, alkyne, a photocleavable moiety, or a photoreactive moiety,
• wherein m is about 1 to about 6000, and
• wherein n is about 1 to about 80;
• the method comprising:
• exposing the linker-functionalized sensor surface to a sensor preparation solution comprising an acid and a surfactant to produce a cleaned linker-functionalized sensor surface,
• exposing the cleaned linker-functionalized sensor surface to a ligand solution comprising a ligand to produce a ligand-functionalized sensor surface;
• and
• exposing the ligand-functionalized sensor surface to a blocking solution comprising an amine to produce a quenched ligand-functionalized sensor surface.