Method of determining fusogenic potential of sperm and use thereof in assisted fertilization and drug screening
AssigneeTechnion Research & Development Foundation Limited
InventorsBenjamin PODBILEWICZ, Nicolas Gaston BRUKMAN, Clari VALANSI
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Abstract
A method of determining fusogenic potential of sperm is provided. The method comprising: (a) contacting a biological sample comprising sperm with somatic cells expressing ectopic JUNO under conditions which allow binding of the sperm to the JUNO; and subsequently (b) detecting multinucleation in the somatic cells, wherein a level of the multinucleation is indicative of the fusogenic potential of the sperm.
Claims20
A method of determining fusogenic potential of sperm, the method comprising:
- (a) contacting a biological sample comprising sperm with somatic cells expressing ectopic JUNO under conditions which allow binding of said sperm to said JUNO;
- and subsequently
- (b) detecting multinucleation in said somatic cells, wherein a level of said multinucleation is indicative of the fusogenic potential of the sperm.
A method of identifying an agent which affects fusogenic potential of sperm, the method comprising:
- (a) contacting an aliquoted biological sample comprising sperm and somatic cells expressing ectopic JUNO under conditions which allow binding of said sperm to said JUNO in presence and in absence of an agent;
- and subsequently
- (b) detecting multinucleation in said somatic cells in the presence and in the absence of said agent, wherein a change in level of said multinucleation is indicative of the effect of said agent on the fusogenic potential of the sperm.
Dependencies are inferred from the claim text.