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US 2026/0133180 A1Published application
Methods for Evaluating Angiogenesis
AssigneeWashington University
InventorsPrincess Imoukhuede, Yingye Fang
Life of this patent
Estimating the term…
Abstract
Various methods for isolating cell populations from adipocyte tissue and/or blood and quantifying levels of vascular endothelial growth factor receptors (VEGFR) on the isolated cell populations are provided. Also provided are methods of evaluating or modifying angiogenic therapy in a subject.
Claims19
A method of evaluating angiogenesis in a subject having or at risk of developing obesity, the method comprising:
- (a) isolating a stromal vascular fraction (SVF) in an adipocyte tissue sample obtained from the subject;
- (b) isolating a subpopulation of cells from the SVF using flow cytometry
- (c) quantifying VEGFR expression on the cell fractions;
- and
- (d) determining an amount of angiogenesis occurring in the subject based on the levels of VEGFR expression on the surface of the cell subpopulation.
2 more elements
A method of isolating different cell populations from a blood sample, the method comprising:
- (a) isolating CD34+ cells from the blood sample;
- (b) labeling the CD34+ cells with cell markers against CD31 and CD146;
- (c) isolating CD34+CD31+CD146− as blood progenitor cells;
- (d) isolating CD34+CD31+CD146+ as mature endothelial cells;
- and
- (e) isolating CD34+CD31+CD146dim as endothelial progenitor cells.
3 more elements
A method of isolating different cell populations from a blood sample, the method comprising:
- (a) isolating CD34+ cells from the blood sample;
- (b) labeling the CD34+ cells with cell markers against CD31 and CD146;
- (c) isolating CD34+CD31+CD146-as blood progenitor cells;
- and
- (d) isolating CD34+CD31+CD146+ as circulating endothelial cells.
2 more elements
Dependencies are inferred from the claim text.