Method for concentrating pathogens or nucleic acid using acid activated bentonite
AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsYong SHIN, Eun Yeong LEE
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AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsYong SHIN, Eun Yeong LEE
Estimating the term…
An embodiment relates to a composition for concentrating nucleic acid or pathogen, a kit for concentration, a method for concentration, and a method for preparing acid-activated bentonite for concentration, the composition comprising acid- and silane compound-modified bentonite and homobifunctional imidoesters as active ingredients. According to the embodiment, it was confirmed that the bentonite adsorbs pathogens and nucleic acids stably and can rapidly elute highly purified nucleic acids. Therefore, the composition can be immediately applied to subsequent analyses such as real-time PCR (qPCR), and can rapidly process large-volume samples using only simple tools, thereby providing both cost efficiency and accessibility. Accordingly, the embodiment can be effectively applied to various analytical methods utilizing nucleic acids and pathogens.
A composition for concentrating nucleic acid or pathogen, comprising acid- and silane compound-modified bentonite and homobifunctional imidoesters as active ingredients.
A method for preparing bentonite for concentrating nucleic acid or pathogen, comprising:
A modified bentonite comprising an amino (-NH₂) or amine (-NHR) functional group on the surface.
Dependencies are inferred from the claim text.