Genic male sterility in watermelon
OwnerNelly VICENTE DÓLERAinventor
Life of this patent
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Abstract
A watermelon plant having a modified Male Meiocyte Death 1-like (MMD1-like) gene, the wild type of which comprises the nucleotide sequence of SEQ ID NO. 2, encoding a MMD 1-like protein comprising one or more modifications in the wild type amino acid sequence of SEQ ID NO. 1, or in an amino acid sequence having at least 90% sequence identity to SEQ ID NO. 1 is provided. When the modified MMD1-like gene is homozygously present, the resulting plant exhibits genic male sterility. Also provided are methods for producing and identifying watermelon plants having the modified MMD 1-like gene. Finally, also provided is a method for imparting genic male sterility a watermelon plant comprising introducing a modification to a MMD1-like gene.
Claims30
A watermelon (Citrullus lanatus) plant comprising a modified Male Meiocyte Death 1-like (MMD1-like) gene;
- wherein SEQ ID NO:
- 2 is the nucleotide sequence of the wild type MMD1-like gene;
- wherein the modified MMD1-like gene encodes a MMD1-like protein comprising a modification in the wild type amino acid sequence of SEQ ID NO. 1, or in an amino acid sequence having at least 95% sequence identity to SEQ ID NO. 1;
- wherein:
- (a) the modified MMD1-like gene comprises a premature stop codon encoding and resulting in expression of the modified MMD1-like protein lacking the PHD_MMD1_like domain;
- wherein the PHD_MMD1_like domain is amino acids at position 551 to 595 of SEQ ID NO:
- 1;
- (b) the modified MMD1-like gene comprises a substitution of a guanine with an adenine at position 12 of SEQ ID NO:
- 2;
- (c) the modified MMD1-like gene has the sequence of SEQ ID NO:
- 4;
- or
- (d) the modified MMD1-like protein has the amino acid sequence of SEQ ID NO:
- 3;
- and
- the modified MMD1-like gene, when homozygously present, confers genic male sterility to the plant.
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A method for producing a watermelon (Citrullus lanatus) plant that exhibits genic male sterility comprising introducing into the genome of a watermelon plant a modified Male Meiocyte Death 1-like (MMD1-like) gene;
- wherein SEQ ID NO:
- 2 is the nucleotide sequence of the wild type ADI-like gene;
- wherein the modified MMD1-like gene encodes a MMD1-like protein comprising a modification in the wild type amino acid sequence of SEQ ID NO:
- 1, or in an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 1;
- wherein:
- (a) the modified MMD1-like gene comprises a premature stop codon encoding and resulting in expression of the modified MMD1-like protein lacking the PHD_MMD1_like domain;
- wherein the PHD_MMD1_like domain is amino acids at position 551 to 595 of SEQ ID NO:
- 1;
- (b) the modified MMD1-like gene comprises a substitution of a guanine with an adenine at position 12 of SEQ ID NO:
- 2;
- (c) the modified MMD1-like gene has the sequence of SEQ ID NO:
- 4;
- or
- (d) the modified MMD1-like protein has the amino acid sequence of SEQ ID NO:
- 3;
- and
- the modified NADI-like gene, when homozygously present, confers genic male sterility to the plant;
- wherein the modified MMD1-like gene is introduced into the genome of the watermelon (Citrullus lanatus) plant by means of mutagenesis, introgression, cis-genesis, transgenesis, or combinations thereof.
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An isolated non-naturally occurring nucleic acid molecule encoding a MMD1-like protein comprising a modification in the wild type amino acid sequence of SEQ ID NO:
- 1, or in an amino acid sequence having at least 95% sequence identity to SEQ ID NO:
- 1;
- wherein SEQ ID NO:
- 2 is the nucleotide sequence of the wild type nucleic acid molecule;
- wherein:
- (a) the isolated non-naturally occurring nucleic acid molecule comprises a premature stop codon encoding and resulting in expression of the modified MMD1-like protein lacking the PHD_MMD1 like domain;
- wherein the PHD_MMD1_like domain is amino acids at position 551 to 595 of SEQ ID NO:
- 1;
- (b) the isolated non-naturally occurring nucleic acid molecule comprises a substitution of a guanine with an adenine at position 12 of SEQ ID NO:
- 2;
- (c) the isolated non-naturally occurring nucleic acid molecule has the sequence of SEQ ID NO:
- 4;
- or
- (d) the modified MMD1-like protein encoded by the isolated non-naturally occurring nucleic acid molecule has the amino acid sequence of SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.