Plastid transformation by complementation of plastid mutations
AssigneePlastomics Inc.
InventorJeffrey Staub
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Abstract
A method of expressing an agronomically or non-agronomically beneficial trait in a plant plastid comprising expressing an exogenous nucleic acid in the plant to produce non-photosynthetic mutant plants, and using callus grown from the mutant plants as recipients for introduction of a construct having a functional copy of the mutated gene and a gene conferring an agronomically or non-agronomically beneficial trait. Embodiments provide for mutations in chloroplast-encoded genes, as well as mutations in nuclear-encoded genes targeted to the chloroplast that are required for photosynthesis, plants and plant parts produced from such methods, as well as kits for performing the methods as described.
Claims11
A method for transforming a plant with a heterologous DNA molecule comprising:
- (a) introducing a heterologous DNA molecule into (i) a recipient homoplasmic non-photosynthetic plant cell comprising plastids with a mutation in a plastid photosynthetic gene DNA sequence to obtain a transformed plant cell containing the heterologous DNA molecule, wherein the heterologous DNA molecule comprises a promoter, a DNA encoding a chloroplast transit peptide (CTP) and a protein having an enzymatic and/or biological activity of a wild-type protein encoded by the wild-type plastid photosynthetic gene DNA sequence, wherein the promoter, DNA encoding the CTP and protein are operably linked;
- (b) exposing the transformed plant cell from step (a) to light sufficient to support greening of a photosynthetic plant cell;
- and,
- (c) selecting or screening for a green photosynthetic plant cell comprising a transformed plant containing a nuclear genome comprising the heterologous DNA molecule from the plant cells exposed to the light in step (b), thereby transforming a plant with a heterologous DNA molecule.
Dependencies are inferred from the claim text.