Method for reprogramming human somatic cells into induced pluripotent stem cells
AssigneeExoneuglia Biotechnology (Suzhou) Co., Ltd.
InventorsYing GUO, Yixing YANG
Life of this patent
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Abstract
A simpler and more efficient method for reprogramming human somatic cells into induced pluripotent stem cells. According to the method, three transcription factors of OCT4, SOX2, and LIN28 are simultaneously transferred into somatic cells, and then reprogramming is performed under the co-induction of four small molecules of Peficitinib, VTP50469, SB203580, and FPFT-2216, wherein the peripheral blood reprogramming efficiency thereof can reach 0.02%, the induction period is only 14-16 days, and the system is stable.
Claims9
A method of reprogramming human somatic cells into induced pluripotent stem cells, comprising the following steps:
- introducing only three reprogramming induction factors, OCT4, SOX2, and LIN28, into the somatic cells, and
- culturing in a presence of chemical inducers to obtain induced pluripotent stem cells;
- wherein the chemical inducers consist of Peficitinib, VTP50469, SB203580, and FPFT-2216;
- and wherein a concentration of Peficitinib is 0.5 μM to 1.5 μM, a concentration of VTP50469 is 0.1 μM to 1 μM, a concentration of SB203580 is 1 μM to 3 μM, and a concentration of FPFT-2216 is 1 μM to 5 μM.
Dependencies are inferred from the claim text.