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US 2026/0132373 A1Published application
Bovine blastocyst like structures and uses thereof
OwnerZongliang Carl JIANG, Yinjuan WANG +3inventors
InventorsZongliang Carl JIANG, Yinjuan WANG, Carlos A. PINZON-ARTEAGA, Jun WU, Yulei WEI
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Abstract
The present invention is directed to method of producing a bovine blastoid and uses thereof.
Claims63
An in vitro method for producing a blastocyst-like structure (blastoid), the method comprising:
- culturing a bovine embryonic stem cell (bESC) or an induced pluripotent stem cell (iPSC) with a bovine trophoblast stem cell (TSC) for a length of time sufficient to observe the formation of a three-dimensional (blastoid) in a cell culture medium;
- and
- incubating a SOX2-positive bESC or a SOX2-positive iPSC with a TSC expressing CDX2 for a length of time sufficient to observe formation of a SOX17-positive hypoblast in a cell culture medium,
- wherein the medium comprises one or more factors selected from the group consisting of a STAT3 agonist, a SMAD2/3 agonist, a Wnt agonist, an inhibitor of the RAS-RAF MEK/ERK-MAPK pathway, a fibroblast growth factor, a PI3K-AKT agonist, or any combination thereof.
1 more element
A cell culture comprising a population of bovine embryonic stem cells in a medium, wherein the medium comprises one or more factors selected from the group consisting of a STAT3 agonist, a SMAD2/3 agonist, a Wnt agonist, an inhibitor of the RAS-RAF-MEK/ERK-MAPK pathway, a fibroblast growth factor, a PI3K-AKT agonist, or any combination thereof.
Dependencies are inferred from the claim text.